human ifitm1 antibody Search Results


caption a7 antibody providers immunogens protein recognized anti ifitm1 goat r d systems  (R&D Systems)
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R&D Systems caption a7 antibody providers immunogens protein recognized anti ifitm1 goat r d systems
Caption A7 Antibody Providers Immunogens Protein Recognized Anti Ifitm1 Goat R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caption a7 antibody providers immunogens protein recognized anti ifitm1 goat r d systems/product/R&D Systems
Average 90 stars, based on 1 article reviews
caption a7 antibody providers immunogens protein recognized anti ifitm1 goat r d systems - by Bioz Stars, 2026-03
90/100 stars
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goat anti ifitm1 antibody  (R&D Systems)
90
R&D Systems goat anti ifitm1 antibody
Hierarchical clustering analysis of GSCs. ( a ) The unsupervised clustering using Ward's linkage method and Euclidean distance individualized H9 HNSCs, H1-H9-ESCs and two groups of GSCs (GSCX-1 and GSCX-2). Note that culture conditions of H9 HNSC do not influence the expression of pluripotency gene samples H9 HNSC-1 and H9 HNSC-2 samples. ( b ) Box plot representation of COL1A1 and <t>IFITM1</t> expression levels between GSC groups GSCX-1 and GSCX-2. The charts show the log 10 expression of relative quantification (RQ) values normalized to the expression of H9 HNSC cell line. ( c ) Box plot representation of COL1A1 and IFITM1 expression levels between bulk tumor groups TX-1 and TX-2. The top edge of the boxes represents the 75th percentile, the bottom edge, the 25th percentile and × the mean. The range is shown as vertical edge.
Goat Anti Ifitm1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti ifitm1 antibody/product/R&D Systems
Average 90 stars, based on 1 article reviews
goat anti ifitm1 antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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Hierarchical clustering analysis of GSCs. ( a ) The unsupervised clustering using Ward's linkage method and Euclidean distance individualized H9 HNSCs, H1-H9-ESCs and two groups of GSCs (GSCX-1 and GSCX-2). Note that culture conditions of H9 HNSC do not influence the expression of pluripotency gene samples H9 HNSC-1 and H9 HNSC-2 samples. ( b ) Box plot representation of COL1A1 and IFITM1 expression levels between GSC groups GSCX-1 and GSCX-2. The charts show the log 10 expression of relative quantification (RQ) values normalized to the expression of H9 HNSC cell line. ( c ) Box plot representation of COL1A1 and IFITM1 expression levels between bulk tumor groups TX-1 and TX-2. The top edge of the boxes represents the 75th percentile, the bottom edge, the 25th percentile and × the mean. The range is shown as vertical edge.

Journal: Oncogenesis

Article Title: A mesenchymal glioma stem cell profile is related to clinical outcome

doi: 10.1038/oncsis.2014.5

Figure Lengend Snippet: Hierarchical clustering analysis of GSCs. ( a ) The unsupervised clustering using Ward's linkage method and Euclidean distance individualized H9 HNSCs, H1-H9-ESCs and two groups of GSCs (GSCX-1 and GSCX-2). Note that culture conditions of H9 HNSC do not influence the expression of pluripotency gene samples H9 HNSC-1 and H9 HNSC-2 samples. ( b ) Box plot representation of COL1A1 and IFITM1 expression levels between GSC groups GSCX-1 and GSCX-2. The charts show the log 10 expression of relative quantification (RQ) values normalized to the expression of H9 HNSC cell line. ( c ) Box plot representation of COL1A1 and IFITM1 expression levels between bulk tumor groups TX-1 and TX-2. The top edge of the boxes represents the 75th percentile, the bottom edge, the 25th percentile and × the mean. The range is shown as vertical edge.

Article Snippet: Antibodies used in this study were goat anti-IFITM1 antibody (R&D Systems Europe, Lille, France), rabbit anti-COL1A1 antibody (Abcam, Paris, France) and mouse β-actin antibody (Abcam).

Techniques: Expressing

Knockdown efficiency of COL1A1 and IFITM1 and effect on cell invasion. ( a ) COL1A1 and IFITM1 mRNA expression was monitored by quantitative reverse transcriptase–PCR in GSC-3 and GSC-9 ( n =2). Significant inhibition of 90% of mRNA expression was observed. GADPH was used as internal control. * P <0.05. Each value is expressed as mean±s.e.m. ( b ) Western blot analysis showed diminution of IFITM1 and COL1A1 proteins expression, β-actin was used as loading control. ( c ) Densitometry of protein level. The bar graph shows relative IFITM1 and COL1A1 protein expression levels expressed as percentage of IFITM1 and COL1A1 expression in control cells ( n =3). We observed >80% inhibition for both genes in GSC-3 and GSC-9 cells, as compared with control cells. ( d ) Representative fields of Boyden cell invasion assay. After 3 days of invasion, cells that migrated across the membrane were fixed and stained with hematoxylin/eosin and were counted in one hundred different fields. The invaded cells were photographed under the microscope at × 100 magnification. ( e ) A significant reduction of invasion is observed in both GSC-3 and GSC-9 cells lines with sh-IFITM1 as well as for sh-COL1A1. The results represent a percentage of invaded cells. Data are presented as mean±s.e.m. from three independent experiments. * P <0.05, ** P <0.01.

Journal: Oncogenesis

Article Title: A mesenchymal glioma stem cell profile is related to clinical outcome

doi: 10.1038/oncsis.2014.5

Figure Lengend Snippet: Knockdown efficiency of COL1A1 and IFITM1 and effect on cell invasion. ( a ) COL1A1 and IFITM1 mRNA expression was monitored by quantitative reverse transcriptase–PCR in GSC-3 and GSC-9 ( n =2). Significant inhibition of 90% of mRNA expression was observed. GADPH was used as internal control. * P <0.05. Each value is expressed as mean±s.e.m. ( b ) Western blot analysis showed diminution of IFITM1 and COL1A1 proteins expression, β-actin was used as loading control. ( c ) Densitometry of protein level. The bar graph shows relative IFITM1 and COL1A1 protein expression levels expressed as percentage of IFITM1 and COL1A1 expression in control cells ( n =3). We observed >80% inhibition for both genes in GSC-3 and GSC-9 cells, as compared with control cells. ( d ) Representative fields of Boyden cell invasion assay. After 3 days of invasion, cells that migrated across the membrane were fixed and stained with hematoxylin/eosin and were counted in one hundred different fields. The invaded cells were photographed under the microscope at × 100 magnification. ( e ) A significant reduction of invasion is observed in both GSC-3 and GSC-9 cells lines with sh-IFITM1 as well as for sh-COL1A1. The results represent a percentage of invaded cells. Data are presented as mean±s.e.m. from three independent experiments. * P <0.05, ** P <0.01.

Article Snippet: Antibodies used in this study were goat anti-IFITM1 antibody (R&D Systems Europe, Lille, France), rabbit anti-COL1A1 antibody (Abcam, Paris, France) and mouse β-actin antibody (Abcam).

Techniques: Expressing, Reverse Transcription, Inhibition, Western Blot, Invasion Assay, Membrane, Staining, Microscopy

Effects of COL1A1 and IFITM1 inhibition on cell proliferation and on neurosphere-initiating capacity. ( a ) Cell proliferation was measured by using a MTS assay. Each experiment was carried out in replicates of six and repeated twice and expressed as mean±s.d. ( b ) Inhibition of COL1A1 resulted in a significant (* P <0.05) decrease of neurosphere-initiating cell (NS-IC) frequency whereas inhibition of IFITM1 expression only slightly modified the neurosphere-initiating capacity. Final cell dilutions yielding 37% of negative wells correspond to the dilution at which there is one NS-ICs per well. Each experiment was performed in duplicate and repeated twice and expressed as mean±s.d.

Journal: Oncogenesis

Article Title: A mesenchymal glioma stem cell profile is related to clinical outcome

doi: 10.1038/oncsis.2014.5

Figure Lengend Snippet: Effects of COL1A1 and IFITM1 inhibition on cell proliferation and on neurosphere-initiating capacity. ( a ) Cell proliferation was measured by using a MTS assay. Each experiment was carried out in replicates of six and repeated twice and expressed as mean±s.d. ( b ) Inhibition of COL1A1 resulted in a significant (* P <0.05) decrease of neurosphere-initiating cell (NS-IC) frequency whereas inhibition of IFITM1 expression only slightly modified the neurosphere-initiating capacity. Final cell dilutions yielding 37% of negative wells correspond to the dilution at which there is one NS-ICs per well. Each experiment was performed in duplicate and repeated twice and expressed as mean±s.d.

Article Snippet: Antibodies used in this study were goat anti-IFITM1 antibody (R&D Systems Europe, Lille, France), rabbit anti-COL1A1 antibody (Abcam, Paris, France) and mouse β-actin antibody (Abcam).

Techniques: Inhibition, MTS Assay, Expressing, Modification

COL1A1 and IFITM1 expression levels in an independent cohort of 30 patients' tumors. ( a ) Box plot representation of the mRNA expression levels. The charts show the log 10 expression of relative quantification (RQ) values normalized to the expression of H9 NSC cell line. The top edge of the boxes represents the 75th percentile, the bottom edge, the 25th percentile and × the mean. The range is shown as vertical edge. ( b ) Kaplan–Meier analysis for overall survival of an independent cohort of 30 GBM patients. Survival distribution of patients with low (group A; n =13) versus high (group B; n =17) expression of COL1A1 and IFITM1 ( P <10 −4 ).

Journal: Oncogenesis

Article Title: A mesenchymal glioma stem cell profile is related to clinical outcome

doi: 10.1038/oncsis.2014.5

Figure Lengend Snippet: COL1A1 and IFITM1 expression levels in an independent cohort of 30 patients' tumors. ( a ) Box plot representation of the mRNA expression levels. The charts show the log 10 expression of relative quantification (RQ) values normalized to the expression of H9 NSC cell line. The top edge of the boxes represents the 75th percentile, the bottom edge, the 25th percentile and × the mean. The range is shown as vertical edge. ( b ) Kaplan–Meier analysis for overall survival of an independent cohort of 30 GBM patients. Survival distribution of patients with low (group A; n =13) versus high (group B; n =17) expression of COL1A1 and IFITM1 ( P <10 −4 ).

Article Snippet: Antibodies used in this study were goat anti-IFITM1 antibody (R&D Systems Europe, Lille, France), rabbit anti-COL1A1 antibody (Abcam, Paris, France) and mouse β-actin antibody (Abcam).

Techniques: Expressing